CHM1054:
Topics in
Bio-organic Chemistry
Time:
Mondays,
1:30-3:30 pm (This may change if there are conflicts)
Room:
DBZ
section - Sidney Smith 621, JS section - CC2110 (UTM)
Instructors:
Professor Deborah B. Zamble
dzamble@chem.utoronto.ca
Professor
Jumi Shin
jshin@utm.utoronto.ca
Announcements:
• The next class will
be March 19th, out at UTM. The best shuttle is probably the one
that leaves at 2:10 (2:05 in the exam week). Class will start
around 3.
• The papers are posted for the second half of the class.
• You should have discussed an abstract with Deborah or Jumi by
the end of March. The proposals are due May 2nd.
Readings
Topics:
(DBZ): Analysis of
biomolecular interactions and their reaction
mechanisms.
- The ribosome -
RNA interactions and amide bond formation.
- Non-ribosomal and
polyketide synthases - multi-subunit interactions and applications.
- Detecting
protein-protein interactions - yeast 2-hybrid methods
- Engineering protein-DNA
interactions - zinc fingers
(JS)
1. De Novo Protein Design
a. Helical structures
b. Zinc fingers, beta-sheet structures,
amyloids, prions
2. Protein Evolution
a. In vitro and in vivo methods
b. Selection assays, library construction
c. Rational vs. nonrational design
Reading:
Items
will be posted on the course web site, cited as library material,
or distributed in advance in class. You will be expected to
read the papers before the lecture and be familiar with the content.
Recommended texts for
background in the major topics:
"Structure and Mechanism in Protein Science", A. Fersht,
Freeeman,
1999.
"Biochemistry," Volume 1, second edition, D. Metzler, Academic
Press, 2001, or any other introductory biochemistry text.
Marking Scheme:
As a core course, the emphasis will be on the application of principles
and methods.
Research
proposal 30%
Class
participation 10%
Mini-presentations 10%
Final
exam
50%
Schedule:
DBZ will
cover from Jan. 8 through Feb. 26, JS will cover March 5 to April
9. The date of the exam will be scheduled during the semester.