Please be patient while the structures in the left frame load. In order to display all of the structures in the tour properly, press 'View' buttons below in order (from 1 to the end).
This is a display of the hydroxylase part of methane monooxygenase from the bacterium methylococcus capsulatus. The hydroxylase component is the largest of the three proteins; it has a molecular weight of 245 Kdaltons. A monooxygenase transfers one oxygen atom from dioxygen onto the substrate. The substrate in this case is methane.
CH4 + O2 + H+ + NADH
CH3OH + H2O + NAD+
These enzymes are found in bacteria, called methanotrophs, that use methane as a source of energy and carbon. The structure of methane monooxygenase consists of three proteins, a reductase, a regulator, and a hydroxylase.
2 binding to the diferrous state to give a diferric
2Fe(III) center containing a peroxide (O22-) ligand
which is probably bridging. This is then converted to water and probably
an Fe(IV)-oxo species which abstracts a hydrogen atom from methane to produce
an Fe(III)-hydroxide. The methyl radical combines with this hydroxide to
form the product methanol.