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Lewis Kay

Lewis Kay

Academic Title: Professor (Cross Appointed), Biochemistry

Phone: 416-978-0741

Office: 1233 MSB

Email:

Research Homepage: http://abragam.med.utoronto.ca/

Research

The research in Dr. Kay's laboratory focuses on the development of NMR techniques for studying macromolecular structure and dynamics and the application of NMR techniques to problems of biological and clinical importance. In particular the research is divided into the following areas:

(1) Methodological Developments. Research is focused on developing 15N, 13C, 2H multi-dimensional NMR spectroscopy to increase the molecular weight limitations currently imposed on protein structure determination using conventional techniques. A second area relates to the development of 15N and 13C relaxation techniques for the study of protein dynamics in solution.

(2) Structural Studies of Proteins Involved in Signal Transduction. In particular, we are studying the solution structures of SH2 and SH3 domains from cytoplasmic tyrosine kinases. These domains bind tyrosine phosphorylated membrane bound tyrosine kinases, implicated in normal signalling and cellular transformation (i.e. cancer).

(3) Cystic fibrosis (CF) is an inherited disease affecting one in every 2000 Caucasians and often leads to death by the age of 30. Recently, the gene responsible for CF was identified. It codes for a large protein called the CF transmembrane conductance regulator (CFTR). The major mutation in patients with CF is a deletion mutation whereby phenylalanine 508 is deleted in the amino-terminal nucleotide binding domain, NBDI of CFTR. Efforts are underway to study NBDI both with and without the deletion mutation in an effort to understand at the molecular level how this mutation results in a dysfunctional protein. 

Selected Publications

See Research Homepage